Website: Revzin Lab
Postdoctoral Fellowship 2004 Harvard Medical School
Ph.D. Chemical Engineering 2002 Texas A & M University
B.S. Chemical Engineering 1998 Wayne State University
Biomedical Engineering Graduate Group
Traditional cell biology techniques monitor large cellular populations and report dominant trends while leaving contributions from smaller cell subsets unaccounted for. Our laboratory focuses on developing novel microsystems for cell cultivation and analysis. The research thrusts in the lab may be subdivided into the following categories.
Cell and Tissue Engineering – Novel surfaces to deliver microenvironment cues to cells. We are employing microarrays of extracellular matrix (ECM) proteins and growth factors to define cell-substrate interactions in a multiplexed fashion. These surfaces are used to identify growth factors that induce stem cell differentiation to a desired tissue type or to determine molecules that protect important cells (e.g. hepatocytes) during injury. In addition, we are developing micropatterned co-cultures in order to study how interactions between different cell types lead to lineage specific differentiation in the case of stem cells or tissue damage in the case of hepatocytes.
Biosensors for Cell Analysis – Biosensing approaches that allow to detect changes in cell phenotype and genotype in the context of local microenvironment. Our vision is to develop micropatterned sensing surfaces that report on changes in cell function in the context of local microenvironment. We are developing a range of biosensors including enzyme-based electrochemical biosensors, antibody-based immunosensors and aptamer-based sensors that may be integrated at the site of the cell to monitor extracellular fluxes of metabolites or signaling molecules.
Point of Care Blood Analysis – Microsystems for multi-parametric blood analysis. We are employing antibody microarrays and microfluidics to develop microdevices for analysis of leukocyte numbers and function. These inexpensive devices allow us to capture multiple leukocyte types (T-cells, monocytes, granulocytes) and to determine function of these subsets (cytokine production) based on a small volume of blood. We are exploring applications of this technology in HIV diagnosis/monitoring, immune profiling of autism and pediatric immunology.
Our work is highly interdisciplinary involving aspects of microfabrication, surface engineering, biomaterials, biosensors and cell/molecular biology.
Shah, S.S., Kim, M., Tae G., Revzin A. “Micropatterning of bioactive heparin-based hydrogels” Soft Matter 2010, DOI: 10.1039/c0sm00771d.
Kim M-S., Stybayeva G.S., Lee J.Y., Revzin A. and. Segal D.J. “A zinc finger protein array for the visual detection of specific DNA sequences for diagnostic applications” Nucleic Acids Research 2010, doi:10.1093/nar/gkq1214
Tuleuova N., Revzin A. “Micropatterning of Aptamer Beacons to Create Cytokine-Sensing Surfaces”. Cellular and Molecular Bioengineering, 2010, doi: 10.1007/s12195-010-0148-5
Tuleuova N., Lee J.Y., Ramanculov E., Zern M.A., Revzin A. “Using Growth Factor Arrays and Micropatterned Co-Cultures to Induce Hepatic Differentiation of Embryonic Stem Cells”. Biomaterials, 2010(35), 9221-31.
Liu, Y., Tuleuova, N., Ramanculov, E., Revzin, A. “Aptamer-based Electrochemical Biosensor for Interferon Gamma Detection” Anal. Chem., 2010, 82(19), 8131–6.
Stybayeva, G.S., Kairova, M., Ramanculov, E., .Simonian, A.L, and Revzin, A. “Detecting Interferon-Gamma Release from Human CD4 T-cells Using Surface Plasmon Resonance”. Colloids and Surfaces B: Biointerfaces, 2010, 80(2), 251-5.
Jones, C.N., Tuleuova, N., Lee, J.Y., Ramanculov, E., Reddi, A.H., Zern, M.A., Revzin A. “Cultivating hepatocytes on printed arrays of HGF and BMP7 to characterize protective effects of these growth factors during in vitro alcohol injury” Biomaterials, 2010(23), 5936-44.
Lee, J.Y., Revzin A. “Merging photolithography and robotic protein printing to create cellular microarrays”. Book chapter in “Biological Microarrays” a volume of “Methods in Molecular Biology””, 2010, 195-207.
Yan, J., Pedrosa, V., Simonian, A.L., Revzin, A. “Immobilizing Enzymes onto Electrode Arrays by Hydrogel Photolithography to Fabricate Multi-Analyte Electrochemical Biosensors” ACS Applied Materials & Interfaces, 2010, 2, 748-755.