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FACILITIES AND RESOURCES
General:
Dr. Passerini’s Cardiovascular Mechanobiology laboratory occupies ~1,000 ft2 in the Genome and Biomedical Sciences Facility at UC Davis. The lab is well equipped for conducting mammalian cell culture, chemical and molecular protocols, and for interfacing with campus core facilities. The lab is equipped for mammalian cell culture with two class II biological safety cabinets, a pair of stackable CO2 incubators, a Beckman-Coulter Z1 dual threshold cell counter, a bench-top refrigerated centrifuge, Nikon inverted and fluorescence microscopes, waterbaths, cryostorage, refrigerator and freezers. General purpose equipment include a chemical fume hood, vacuum concentrator, microcentrifuges, electrophoresis equipment, wet and dry baths, utility oven, pH meter, top-loading and analytical balances, pipetters and pipet-aids, hot plates, stirrers, vortexers and shakers.
Major Instruments and Technologies:
- Eppendorf Mastercycler ep realplex real-time PCR system
- Nanodrop ND-1000 spectrophotometer
- Bio-Tek Synergy HT plate reader
- Agilent 2100 Bioanalyzer with electrophoresis and flow cytometry modules
- Optiplex GX620 computer workstation with software for the analysis of genomics data
Additional resources for microscopic imaging, microfabrication and flow cytometry are available through collaborations with other department faculty.
Core Facilities:
The Genome Center at UC Davis maintains core facilities in DNA technologies and Expression Analysis, Proteomics, Metabolomics and Bioinformatics. Also located in the Genome Center is the state-of-the-art Center for Molecular and Genomic Imaging.
Soft photolithography equipment is available in a departmental core facility including a spincoater, mask aligner, and plasma hoods. Furthermore, the College of Engineering hosts the Northern California Nanotechnology Center, a world class center for the synthesis, fabrication, and analysis of nanoscale devices and materials, that provides microfabrication resources to investigators.
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